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2Fat2EatThat

DIY LACTOBACILLUS SERUM.

Posted (edited)

Hey growers, 

 

This post is the first of a few I'll make related to creating natural products with simple ingredients that can be used not only for the plants we grow here but gardening or farming in general. 

 

They contain methods and associated information from various websites, some foreign language, I've visited whilst browsing the growing globe and as such would like to state the following -

 

I do not have copyright on any of this information. 

 

I did not develop any of the techniques or products listed. I do not have any ownership over them and will not take any credit or gain from them. 

 

             -------------------------------

 

With that done and out of the way here is the first recipe to make beneficial bacteria. 

 

I'd like to state the recipes are all rather large scale but are simple to scale down and work just as well. 

 

This is from an Asian eco farming website. 

 

To make a Lactobacillus Serum. 

 

This is the workhorse of the beneficial bacteria we’ll be discussing here. We use it for everything! Foul odors, clogged drains, cheaper pig/chicken/etc farming, aquaculture, the applications are amazingly diverse. Learn how to make and use this and you will have a powerful tool in your farming arsenal.

How to Make: 

Get container, fill halfway with rice-wash. Rice wash is the water leftover when you rinse fresh rice. For example, go buy rice, whatever kind, bring it home, put it in a pot with warm water, swirl it a bit and then drain the [now milky colored] water. The water is now a rich source of carbohydrates. In this step, you can substitute rice with another carbohydrate source if you don’t have rice, as long as it is complex (don’t use simple carbohydrates like sugar, honey, syrup, molasses, etc). You can use wheat, barley, kinoa, other carbohydrates as the base to make your carbohydrate wash. This wash will attract microbes from the air, among them lacto bacilli. 


Cover loosely and let stand for a couple days to a week

When is it done? When you see a light film on top (molds) and it smells a little sour and forms 3 layers. This is indicating the rice wash is infected with various microbes. This happens more quickly in warm temperatures because microbes are more active. Thus it is all relative since we don’t do this in controlled laboratory conditions. 

The layers are distinct

Top layer: floating carbohydrates leftover from fermentation and possibly molds

Middle layer: Lactic Acid and other bacteria (cheese buffs will recognize this as a makeshift “rennet”). We will use this layer. 

Bottom layer: Starch, byproduct of fermentation

Extract the middle layer using a siphon. This layer contains the highest concentration of lactic acid bacteria and lowest concentration of the unneeded byproducts

Get a new container, larger than the first. Take the extracted serum from the last step and mix it with 10 parts milk. By saturating with milk (lactose), we dissuade other microbes from proliferating, leaving L. bacilli. E.G. if you have 1cup of the serum, mix it with 10cups milk. 

TIP: The best milk to use in unpasteurized natural milk. However, any milk will do, even powdered milk. In our experience, the best is unpasteurized natural but just use what is available. We just want to saturate with lactose to promote L. bacilli bacteria. 

You want to keep this stage anaerobic as much as possible. You can use something like rice bran, barley bran, wheat bran, etc sprinkled on top of the milk. I use a sealed container with a one-way valve.  

Note: Beware of bubbling during this phase. It can lead to overflows if you filled to near the top. It can go through the one-way valves so keep an eye on it and don’t do this step around nice things.

After about 1 week (temp dependent), you’ll see curds (made of carbohydrate, protein, and fat) on top of the milk. The water below will be yellow colored – this is whey, enriched with lactic acid bacteria from the fermentation of the milk.

NOTE: Microbes like L. bacilli are more active in warmer temperatures. The curds you see are a byproduct of the fermentation process. Fermentation is generally associated with microbial processes under anaerobic(no oxygen) conditions. Now, L. bacilli is a facultative anaerobe, that is it can live and work with or without oxygen, but less competition in anaerobic conditions.

The water below(whey+lacto) is the good stuff. You want to extract this. You can either skim the curds off the top, pour through a strainer, or whatever other methods to accomplish that

NOTE: Remember the curds, or byproduct of milk fermentation by L. bacilli, are great food. They are full of beneficial microbes like L. bacilli. Feed the curds to the soil, compost pile, plants, animals, humans – whoever wants them! They are full of good nutrients/microbes. No waste in natural farming.

To preserve at room temperature, add an equal part sugar/molasses to the serum. So, if you have 1L of serum, add 1kilo sugar or 1L molasses. Otherwise store in fridge to keep.

Example Recipe:

1 L rice wash

 

add 10L Milk

After rice wash and milk remove curds – around 1L

Left with 10L pure LAB (lactic acid bacteria)

add 10kg sugar or 10L molasses

= 20 L stabilized lactic acid bacteria serum

 

Before using, first mix 1:20 with water. 1 part serum to 20 parts water. Then follow instructions below:
 
Plants – Growth Aid:  

When added to water for plants, nutrient uptake efficiency is increased, which increases growth! 

Improves growth of plants when applied as foliar spray and soil drench. Improves their efficiency in uptaking nutrients so naturally, growth is enhanced. With the use of these microorganisms, the nutrients you spray or drench to feed your plants become more bio-available and easily absorbable by the plants. Technically, you can say that plants do not use organic nutrients directly. Microorganisms convert organic nutrients to their inorganic constituents which the plants utilize. Utilizing microbes, you will notice better plant growth and health. 

 
Disease Resistance: 

This is a consequence of the increased efficiency of nutrients. More nutrients available at smaller metabolic cost. 

Lacto suppresses harmful bacteria in food/water that animals consume, enhances their gut flora so that line of defense is working optimally, etc.

 Aid Compost: 

Mix 2tbsp/L and spray on compost pile to improve decomposition. This is a huge topic that will be expanded upon in another post.

 Aid Organic Fertilizer: 


Add 1-2tbsp per gallon water-nutrient solution. Lacto consumes organic nutrients making them bio-available to plant roots.

Plants don’t use organic fertilizer! Microbes break it down to inorganic constituents, and plants take those up. This product makes that process more efficient.

               ----------------------------

As I mentioned at the start this is the first of several recipes from natural farming among others that I will be starting and diarising with photographs as my own first step into this interesting world. 

 

So plenty more fun to come, some techniques are relatively simple but some are longer term and more difficult whilst still being essentially simple and natural. 

 

Until next time, take it easy folks and keep those roots happy! 

 

 

 

 

 

 

 

Edited by 2Fat2EatThat
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Posted (edited)

Like this?

large.5d0268374ce18_day3(2).jpg.9b6b4177day4.jpg

 

 

What do you use it for mate?

Edited by Jimboo
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Posted (edited)

That looks like the stuff buddy yeah! lol

 

Ive never actually made it yet jimboo, I couldn't grow for along time as life beat me down but I did a lot of reading for my return and am now able to experiment at last. 

 

So I return the question - what do you do with yours?! 

 

Its nothing spectacularly ground breaking but just love the idea of making my own. Some of the others I have details and will post are - 

 

BIM - Beneficial Indigenous Microbes. 

 

FST - Fermented Sprout Tea. 

 

Ginger Garlic Extract. 

 

Various Fish based products.

 

CALPHOS - Calcium+Phosphorus obv. 

 

A Grow N based feed. 

 

A fruit based Bloom feed. 

 

Activated biocharcoal using some of the above recipes to charge it with goodness. 

 

So yeah not ground breaking but fun, effective and natural which is always the best way if possible to my mind. Some take time to do which is another draw to keep my simple mind amused. 

 

 

 

Edited by 2Fat2EatThat
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So I return the question - what do you do with yours?!

The only thing I have been using it for is making nettle and comfrey teas, It just does away with the smells unbelievably well, I did foliar feed my seedling with it,but I have no evidence that it did any good, certainly didn't harm them.I'm new to using it as well but just that odour control on the tea means I will make it again

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Cool I bank on the smell of the teas to mask the smell of budding plants.

:taz:

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Posted (edited)

@2Fat2EatThat I think you should skip to making Various Fish based products. I will be looking forward to that :yes:

Edited by Jimboo
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Made this during first grow. Think I used for foliar spray and a little in compost tea. Surprised how easy it is make. 

 

You can drink it too (diluted). I didn’t drink mine. Maybe after I’ve made it a few times.  

 

 

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I've made this too recently, not used it on my indoor garden yet but I have an experiment plant outside that is getting fed this. Not harming it yet that I can see, not sure if it's helping at all either lol

 

I've tried it in my dishwasher too cos it stinks sometimes, seems to damp down the smell, although it comes back soon.. probably need to clear the drain out :puke:

 

Always interesting to try these things out, I'm subbing as I'm interested in your other concoctions too dude, thanks for sharing:)

 

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Who wants the  Various Fish based products. next? with pics of course    me me me

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