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> Making feminised seeds..........., Anyone ever tried it?
Bad Penny.
post May 25 2009, 01:31 PM
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Colloidal silver seems the easiest and surest route thus far,I am going to make a generator,I might also dabble in Giberellic acid as I can get it in powder form sent over from Asia,already got someone on the case for me checking out prices,I think its a tenner a sachet unsure.gif

http://en.wikibooks.org/wiki/Marijuana_Cul...Producing_Seeds
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Bad Penny.
post May 25 2009, 01:49 PM
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Soma feminises his seeds in a different way,all good stuff though wink.gif

Feminized seeds
It is possible to use a combination of cloning and "shocking" plants to get them to produce feminized seeds.[5] A clone will retain the same sex throughout its life, so if a female plant is cloned, its clones will also be female, precluding reproduction.

Some claim (particularly vendors of feminized seeds produced with certain methods) that while environmental stresses have been used to create pollen bearing male flowers on female plants- known as 'hermaphroditing' or 'hermying', this method is not preferred when creating feminized seeds; due to those plants most likely to revert to seed making being the ones which hermie soonest; hence passing on the genetic trait of instability of gender - desirable in the wild but not in cultivation.

Spraying selected leaves, branches and in cases where large amount of seed desired whole plants with colloidal silver solution has become a preferred method since the colloidal silver suppresses ethylene production in bud sites, stimulating male characteristics. Gibberellic acid has been used frequently; but is harder to find than colloidal silver, which involves nothing more than a small wall d.c. power supply and two pieces of solid silver jeweler's wire, or 99.999% silver coin. A method used by organic growers and promulgated by the famous Cannabis breeder Soma, is called 'Rodelization', or letting unpollenated female plants live several weeks longer than the normal harvest time. In such plants a hermaphroditic trait will self express in effort to continue the genetic line; the fact this method utilizes auto hermaphroditic traits which could contribute to instability in a plant's genetics is offset by grower observations that the tendency to auto-switch sex is not great in plants grown from seeds made this way, and the fact that it occurs naturally without effort on the part of the cultivator.

However, other cultivators claim that the genes responsible for hermaphroditism are present and may be activated under stress from any of the above methods and that once activated can be passed to seeds regardless of how it was activated. Previous theories would not have allowed for the passing of genetic alterations an adult has acquired after birth but there is evidence that when a gene is activated in a mother after her birth that expression can be passed to offspring.

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mudkipz
post May 25 2009, 01:54 PM
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If you're talking about an outdoor grow me reckons it'll be easier to use chemicals...unless you really wanna fuck around covering plants and such...Again, good luck with it, be interesting to see how it pans out, maybe your own seed company....


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Bad Penny.
post May 25 2009, 01:59 PM
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I am doing these outdoors mud,I am going to try both colloidal and giberellic,as for my own seed company let me tell you a little story,very early 1990's i was based in Pakistan NWFP right on the edge of the khyber pass,place called peshawar,I travelled a lot around the region and over the border into afghanistan,a lot of things were happening in the region at the time.Anyway,there used to be a small advert in the back of VIZ magazine,a company called sunlight systems was selling cannabis seeds ph34r.gif I could have had kilos of seeds as I had a number of contacts in the area and had to get off the beaten track often,I mused over it once,and my best friend Tony(Who still is my best mate actually)said "Cannabis seeds?What fuckers foing to be daft enough to buy cannabis seeds never mind f**king sell them" famous last words rofl.gif
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mudkipz
post May 25 2009, 02:04 PM
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If only... you could have been namkha!
I would love to go to the hash regions around kush, just worried about how war-torn it can be nowadays...
I guess nowadays the next best thing is making your own, I'm so tempted to grow some kush or sheberghan and bubble bag all of it, make some real fucking hash...


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Bad Penny.
post May 25 2009, 02:07 PM
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Its not difficult to get your hands on any landrace mate,all you need is a passport wink.gif Like I said,I will try the femmie seeds this year,the more I read about it the more positive I am that the techniques will work,theres hundreds of pages about it scattered all over the net so I know theres substance to the techniques,I just read about one guy who has used CS for the last 8 years with great success wink.gif
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Bad Penny.
post May 25 2009, 02:20 PM
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Just thought I would stick this link up as theres an excellent post by grimweeder on making colloidal silver wink.gif

http://www.uk420.com/boards/index.php?show...mp;hl=colloidal
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Delta2012
post May 25 2009, 04:08 PM
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I've been getting female pollen and resulting seeds are all proving female thus far from 5 strain crossings in the past 11 months and must be about 8-9 plants now, i've grown of my own femmed seeds, among fresh strains i've purchased in the same time, also getting onto F3 with my Easy Sat x Thai/Haze/Skunks, with the Thai Skunk female pollen.

I don't use Silver Thiosulphate or Gibberelic acid though, nor light poisoning.

I hardwire the dioecian survival reflex through stress, after a sacrificeable plant is about 2-3 weeks into budset, (Obviously some plants are faster/slower than others, but roughly 2-3 weeks after budset.) and the main cola has a couple of hundred hairs, (Some strains have huge calyxes and so fewer hairs, plants i've done thus far had profuse calyx clusters.) and looking through the hairs the bud looks about the size of the end segment of my little finger(I've big hands so somebody with small hands would likely be looking at their index finger, last segment.), by rubbing out all the hairs on the main cola, squishing the bud head but not damaging it too much, about the same firmness as rolling a cigar between forefinger and thumb, maybe a little more.

Doing this will cause male flowers at all the branching and internodal points one usually gets male pods emerging.

I've done this to a few plants so far, works a treat.

First was my first KC36 and i did so by accident, crushed all the hairs of the main cola and **BANG**, 2 days later pods had appeared, grew very fast and they popped with some energy as well as very quickly compared to males and the couple of genetic hermies i've unfortunately had in times past.

I think this is because the 12/12 regimen has had the plants phytochrome levels at peak plateau for a couple of weeks, i do it at about 4-5 weeks into 12/12 and it usually takes about 2 weeks for a nice budset, so when the plant has the main female sex organs at the main cola essentially destroyed, the flower switches directly into the dioecian survival reflex, as opposed to males and some hermies develop flowers a bit slower. A fast and acute trigger during flowing, not a build up to hermaphroditism.

When i've done this, the pods appear double quick time, first i saw it with my KC36, i was awestruck at how they just developed practically overnight and shot pollen at force within days.

I've actually got 2 of my Easy Sat x Thai Skunk growing now, almost sexed although i know they will be female, one i intend vegging another couple of weeks and the slower one is going 12/12 in a few days as she will be sacrificed about 2nd week of July when i crush her main cola to get feminised F3's and hopefully some quite potent but fast Sat's expressing as phenotypical.

Going to reveg my Himalayan Golds as well, getting some sinse bud first, but one will go the same way and think i may reveg and see whether the stress follows through or after a reveg it reverts to female again, something the mad scientist in me has wondered a few times so i want to see the result.

I can qualify that plants from these seeds i've made this way are proving consistantly female, no males and not one hermaphrodite, so far.


Give it a try! Even if you only do this with bagseed to see the resuts, it'll be interesting, female pollen, no chem's! spliff.gif

Works well with me. cool.gif

Paxus. yinyang.gif

[Oh yeah, on average the plants i've stress herimied have been 10-12 week finishers, so faster finishing plants will likely have faster budset etc, so you'd want to do the cola crushing a couple of weeks earlier etc.]

This post has been edited by Delta2012: May 25 2009, 04:14 PM
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Bad Penny.
post May 25 2009, 04:14 PM
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Thanks for that Delta,very interesting indeed but I am looking for a large quantity of seed,maybe 1000+ beans,at the moment its easier for me to go the chemical route which is the route the seedbanks normally take,but I will certainly follow your instructions in the future,I wonder if it will work with outdoor plants?I could probably rough-up a single bud and test it out unsure.gif You have certainly given me food for thought,I salute you sir and once again thank you very much.Its all a learning process isnt it ph34r.gif
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Delta2012
post May 25 2009, 04:18 PM
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Indeed, if you're talking a mass endeavour, chemical would seem the more efficient. wink.gif

I like to let people know about this stress hermie method though, it's great for the small grower. cool.gif

Aye, life is a learning curve, as i always say. spliff.gif

Paxus. yinyang.gif
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BluePixie
post May 26 2009, 06:52 AM
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Hey Bad Penny,

I've attempted to self a SK1 mum I had using CS.

I made the CS solution using a 9v DC power supply, a couple of croc clips, some silver wire and distilled water. I was growing 11 clones in a minimal veg sog (they had about 6 days veg), and sprayed 1 clone daily with the solution from about 3 days prior to 12/12.

Results: The sprayed clones was a runt compared to it's sisters, but produced female buds. It also produced a single ball sack about 4 weeks into flower, (at which point I stopped spraying), and then went onto produce a shit load of stealth nana's about 6.5 weeks into flower. As I didn't think any seeds produced at this point would have time to mature I binned it to avoid pollination.

If I try this again I will spray the treated plant from about 3 -4 weeks prior to going into flower with the hope of getting the male expression earlier and therefore a chance for viable seed.

Having read around about the process and I came across a couple of pointers - 1) selfing is not particularly desirable due to likely inbreeding depression in the offspring. 2) The clone chosen to donate the female pollen should be throughly stress tested to ensure that it only herms under chemical treatment and not under under typical grow-room stressors. This should reduce the chance of herm tendencies in the offspring.

Good luck with your endeavors! cool.gif



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Grimweeder
post May 26 2009, 09:08 AM
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blue pixie got it in one, you need to spray the sts or cs on the plants around 3-4 weeks before 12/12 this will make the plant produce mainly nanas or pollen sacks an not bud up. an also ensure your chosen female to be reversed has been well stress tested otherwise youl probably end up with hermies. the key is selecting a good plant to reverse.
its quit simple an relatively easy to accomplish viable seeds as long as you do it properly an dont cut corners unless absolutely necessary.
good luck with it.




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GREENDUP
post May 26 2009, 02:29 PM
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How about combining !Take cuttings from the plants that are out, back home, and try each method including force flowering outside or inside.Then take your pollen back out to the plants outside when they are ready?If you are unable to use your home as a growsite at all how about leaving one plant in a pot and when it comes to flowering for a couple of weeks turn it around 180 degrees every time you visit it ?or place in the shade?mine auto pollinate at 2nd or third node up half way along the branch, not high up enough to make lots of seeds on its own. stoned.gif


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Bad Penny.
post May 30 2009, 02:00 PM
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Apparently theres a seedbank currently working on feminising the original cheese,anyone heard of it? unsure.gif
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Stonehenge
post May 30 2009, 02:14 PM
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QUOTE (Bad Penny. @ May 30 2009, 03:00 PM) *
Apparently theres a seedbank currently working on feminising the original cheese,anyone heard of it? unsure.gif


Hiya BP.. don't know about that unsure.gif but I found a thread by oldsmoker where he produces fem seeds...Here you go! Happybrother...

Worth a look...


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